Column:

Polar-Silica, 5 mm, 120 Å, 4.6 x 250 mm                   

Mobile phase:


Time(min):
B%:

A: CH₃OH : H₂O : CH₃COOH : C₆H₁₅N = 85 : 15 : 0.45 : 0.05 ( v/v )                       
B: C₆H₁₄ : (CH₃)₂CHOH : A = 20 : 48 : 32 ( v/v )
0    20  35  36  41
90  70   5   90  90

Flow rate:

1.0 mL/min

Temperature:

40 ℃

Detection:

ELSD

Injection volume:

20 µL

Sample:

4 mg/mL in CHCI₃ /CH₃OH = 2 : 1 (v/v)